Save the results of the Protocol in a data (. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. This integrated system provides users with a multi. For life sciences research, the system is well suited. book Page 1 Wednesday, July 7, 2010 2:09 PMon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. We would like to show you a description here but the site won’t allow us. Choose cables that meet the requirement described in this appendix. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. -49. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C) AccuBlue HiRange kit. Molecular Devices. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMD Spectramax iD5 Plate Reader. This problem has been remedied by the introduction of Molecular Devices PathCheck Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Our absorbance plate readers feature our PathCheck Sensor technology and. Histamine H1 is an endogenous receptor in HeLa cells. 0. Table of Contents. View the Molecular Devices websiteFigure 2. UV/Vis Photometric Performance: Wavelength Range. 2. Description 1. Optimal instrument. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. -36. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. 1. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. FP assay do not perform well with protein:protein interactions. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The following document is intended to demonstrate setup of this instrument. Prepare your i3/i3x instrument for shipment. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent). Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. Molecular Devices SpectraMax M5 Plate Reader Repair. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. It offers five preferred detection modes, wide range of applications, low volume. +1 800-635-5577. 1700 +1 800. We would like to show you a description here but the site won’t allow us. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Molecular DevicesSpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). #I2511) • Assay diluent – may be cell culture mediumThis process should take about 3 - 5 minutes. 3. After running the LM1 Plate protocol, not all values in the values in Results sections are displayed; what should I do? This is a SoftMax Pro software problem rather than anything specific to the LM1 plate, protocol or reader. Detection cartridge modularity allows for easy addition of new read modes within minutes without. This document is provided to customers who have purchased Molecular Devices, Inc. Fortunately, Molecular Devices offers a system with variable bandwidth that can be specific and sensitive at the same time and is suitable for assay optimization, so you do not have to choose. #R7271) Akt1-PKBa 激酶 (Upstate Cat. D 1 1. SpectraMax® iD5. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. the system provides automation for up to 50 microplates for easy walkaway automation. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. 4. StakMax Microplate Handling System. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. on the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. Please refer to the Obsolete Microplate Reader Models for more information. D. 2. It has patented optimization, IQ/OQ/PM services,. 100 readings per well gives optimal Z’ values for this assay. The following document is intended to demonstrate setup of this instrument and provide representativeMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Call 516-939-0888 to check current. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. No accessories are needed and SoftMax® Pro. -43. 2. Lower end of DNA standard curve. Transcreener ® ADP. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Download PDF. We therefore gathered salient. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Note: In this user guide, all references to SpectraMax Multi-Mode. EUROPE. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Home; Knowledge BaseThe introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. 0A-Sunnyvale, CA-Made in USAIntroduction. This document is provided to customers who have purchased Molecular Devices, Inc. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. , contact Molecular. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. $2,264. Features. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. , October 25, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of SpectraMax® M5, a five-mode. If no values are present, the water constant values are missing from the NVRAM. ® reader to the computer. If depot service is the best option for you we will assist you with logistics to help you keep. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. It has patented optimization, IQ/OQ/PM services, validation tools, and compliance software. The donor and acceptor can be used to label a variety of biomolecules, for. SpectraMax M-series Microplate readers. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The fluorescentMolecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). View the Molecular Devices websiteThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. 11/22 2295B. FlexStation® 3 Multi-Mode Microplate Reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Principle of the HTRF IP-One competitive binding assay. 4. Fluorescence Microplate Reader Comparison Chart. -39. With optimized reagents, option to operate the. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). system setup and calibration are controlled from within softmax ro psoftware. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. . 312 gain calibration failed. Products : SpectraMax M2/M2e reader, FlexStation II (Obsolete), FlexStation 3 reader, SpectraMax M5/M5e reader. The opaque walls prevent stray light from being detected from neighbouring. To support these assays, we provide application notes as well as. 4. View the Molecular Devices websiteMolecular Devices’ SpectraMax® L Luminescence Microplate Reader is a dedi-cated reader with an extended dynamic range of more than 8 orders of magnitude. 1. SpectraMax Injector Cartridge with SmartInject™ Technology. Various data transformation steps in the software are discussed, such as normalizing the GFP. Enzyme dilution curves were performed for Lck, a tyrosine kinase withThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. 0 out of 5 $ 5,999. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. book Page 1 Wednesday, July 7, 2010 2:09 PM. . NORTH AMERICA. The SpectraMax M2 and M2e are versatile and reliable. 2. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Select the Driver tab. The chamber. Plate chamber design of the SpectraMax category 1 microplate readers. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 302 low ref light. Sensitive fluorescent quantitation of DNA with the Quant-iT. ©2013 Molecular Devices, LLC • 8/13 • Printed in USA • PN: 0120-1521. Wait 20 seconds. handling system from molecular devices. The following document is intended to demonstrate setup of this instrument. Printed in USA. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next- BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. View the Molecular Devices websiteThis version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Pleasanton, CA, USA. Specifications subject to change without notice. Table of Contents. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre-configured protocol provided for customers using Molecular Devices’ SoftMax. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. 3. View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seWe would like to show you a description here but the site won’t allow us. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. It has a cuvette port, spectral scanning, and up to six wavelengths per read. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. For SpectraMax L or LMax II, LMax II 384 - no adapter used. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. 3 or greater. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Database contains 2 Molecular Devices SpectraMax M5 Manuals (available for free online viewing or downloading in PDF): Operation & user’s manual . Simple, homogeneous workflow minimizes cell handling. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. The chamber. Molecular Devices, LLC Subject: SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. 7 are achievable. Both microplate and cuvette ports can be qualified. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Turn on Plate Reader and wait. The drawing shows a standard microplate of 15 mm height covered with a lid. CHO M1WT3 antagonist IC50 curves. 635. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. #6590-0118) Filter 665/10 (Molecular Devices cat. Molecular Devices Spectramax M 5 Manual, 2000 Vw Beetle Workshop Manual, Proton Supplier Quality Manual, Beltronics Rx65 Red Manual, Vfr 400 Owners Manual, Hgv Mot Testing Manual, Prostart Manual Installation. 5. 2. ATP standard curves read on SpectraMax microplate readers. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. 1. 5577. 1. . i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3 Validation Plate 0200- Cuvette Absorbance SpectraMax Plus 384, ABS Plus, M2, M2e,. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Bookings are free, but you will need to use iLab to book. A highly sensitive instrument with reduced noise and low cross talk, it is also a configurable system with up to 6 channels (photomultiplier tubes) and 12 injectors. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. If values are present, then. Pick the best speed, sensitivity, and price for your needs. It does not work as expected. e. Fifteen different automated tests are provided. If the computer is connected to the Internet: Select Search Automatically for updated driver software. The KeySpan USA-19HS is often required with new Windows 7. i3. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Instrument settings used with the SpectraMax M5 Microplate Reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. View the Molecular Devices websiteMolecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Figure 3. Other technologies such as imaging. Description: Molecular Devices ’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Molecular Devices has collaborated with various assay partners to optimize andFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Additional information is also available in the User Manuals. The StakMax system is an easy-to-set-up, powerful, and reliable stacking solution for. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Materials. Restart the system. industry-leading data acquisition and analysis tool SoftMax Pro, the SpectraMax i3x Detection Platform is the total solution for all your research needs. This document is provided to customers who have purchased Molecular Devices, Inc. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. It is possible to search for the Overlord device driver for the instrument of your choice. com A. We would like to show you a description here but the site won’t allow us. Turn the reader on and secure the USB cable. 0 out. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 4. All results should now be displayed. SpectraMax® M5. Molecular Devices Spectramax M5 Microplate Reader; Related products. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Molecular. The built-in micro-volume sample port allows for working with sample volumes as small as 0. IMAP technology is based on. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. performs within its specifications. Lift up the lid over the chamber to insert or remove a cuvette. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. You should get comparable results from top and bottom reads using EC50's to compare reads. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Home; Knowledge BaseMolecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Manufacturer: Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The chamber contains springs that position the cuvette in the proper alignment for a read. Spectral Range. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Cables. minutes and begin reading microplates with seven mouse clicks. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This application note demonstrates the validation of the Molecular Devices SpectraMax® M5/M5e & FlexStation® 3 microplate readers for use with the Transcreener® FI Assays. Multimode Reader Comparison Chart. +1 800-635-5577. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Order Now. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. 2018. On-site Repair Service available nationwide for Molecular Devices SpectraMax M5 Plate Reader's. assays and enable users to choose the detection format they prefer. The system provides automation for up to 50The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Detection modalities are shown in Table 1-1. Molecular Devices User Guide. SpectraMax M5 microplate reader. STAKMAX. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. pda) file, close, and then re-open. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. SoftMax Pro Software can collect data from one or more microplates orSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 We would like to show you a description here but the site won’t allow us. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Select Update Driver. SpectraMax M-series Microplate readers. SpectraMax Glo Steady-Luc Reporter Assay Kit Luciferase measurement for your microplate reader assay KEY FEATURES • Glow-type luminescence provides an extended signal time window Molecular Devices. 5. Learn about the SpectraMax® M5 Multi-Mode Microplate Reader, a versatile instrument for bioresearch and drug discovery applications. Multimode Readers Comparison Chart. Supplier: Molecular Devices. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. HEALTH AND SAFETY CONSIDERAIONS The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. (“Molecular Devices”) equipment, software, reagents, and consumables to use in theWe would like to show you a description here but the site won’t allow us. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. EarlyTox Live Cell Assay (6. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. on the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Homogeneous Time-Resolved Fluorescence (HTRF) . Stray light. Homogeneous Time-Resolved Fluorescence (HTRF) . x and earlier. View the Molecular Devices website. HTRF-compatible SpectraMax Multi-Mode Readers. 1 ng/mL to 50 pg/mL (r2 = 1. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 305 reference level saturation. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. view more . Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 7°C and the bottom to 36. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Read More. Figure 2. SpectraMax Instrument optical verification > IQ/OQ/PQ validation protocols for Molecular Devices microplate readers and washers > In-field reader Performance Certification. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. 2. Monday to Friday 8am - 5pm GMT. Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. For top. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except asIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput screening. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. This document is provided to customers who have purchased Molecular Devices, Inc. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 3860 N First Street San Jose,CA 95134 USA +1 408. 2. 2. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Check: In the data table create two new columns and add the following formulas: !Pathchecklm900. SpectraMax Gemini EM & FLEXstation (bottom read) 1. i3, M3, M4, M5, M5e, SpectraMax L, FlexStation 3 0200-6186: Cuvette Absorbance Validation Set SpectraMax. ECvalues were within one-half log and Z factors at EC. Step 1: Open the SoftMax Pro IMAP FP protocol. The system provides automation for up to 50 The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 9. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Optimal instrument settings are the same for all three readers. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The bottom read fluorescence mode should be used. Using a cell-based standard curve and a five-minute incubation, we observed a limit of. SpectraMax Glo Steady-Luc Reporter Assay Kit Luciferase measurement for your microplate reader assay KEY FEATURES • Glow-type luminescence provides an extended signal time. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Microplate Readers include the M3, M4,. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L. 72 MB.